Circular RNA ITCH downregulates GLUT1 and suppresses glucose uptake in melanoma to inhibit cancer cell proliferation. What are the known protein-protein interactions of GAPDH GAPDH interacts with other proteins to regulate nuclear translocation and GAPDH catalytic activity. It is also useful for cell visualization in microscopy. Because GAPDH is often highly expressed throughout different tissues and cell types, GAPDH expression is useful as a control for Western blots and RT-PCR. m6A methyltransferase METT元-induced lncRNA SNHG17 promotes lung adenocarcinoma gefitinib resistance by epigenetically repressing LATS2 expression. For this reason, GAPDH is widely used as a loading control for protein normalization in Western blotting. Curcumol enhances cisplatin sensitivity of gastric cancer: involvement of microRNA-7 and the nuclear factor-kappa B/snail family transcriptional repressor 1 axis. GAPDH Western Blot Most recent answer Mateo Glumac University of Split 15th Feb, 2021 I found some westerns on the internet with double bands for GAPDH in Rat and Mouse brain homogenate but. KLF4 regulates TERT expression in alveolar epithelial cells in pulmonary fibrosis. KLF4 downregulates FGF21 to activate inflammatory injury and oxidative stress of LPS-induced ATDC5 cells via SIRT1/NF-?B/p53 signaling. Solve your western blot problems with these troubleshooting tips, covering common causes of no signal, high background, multiple bands, and more. OriGene offers high quality of Anti-GAPDH TrueRAB antibody (Loading control). Preliminary data indicates that the GAPDH antibody- loading control ab8245 recognizes the monomer (36 kDa) and also the dimer forms of GAPDH, but not the tetrameric form of the protein. GAPDH antibody, is usually used as a loading control antibody for Western Blot to normalize the levels of protein detected by confirming that protein loading is the same across the gel. There is no cross-reaction with GAPDH from yeast. Publishing research using ab8245? Please let us know so that we can cite the reference in this datasheet.Īb8245 has been referenced in 3490 publications. GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits.
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